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줄기세포배양액의 주름개선 효과 기작 밝힌 논문 발표
작성자
셀인바이오
작성일
2016-07-04 17:41
조회
522
줄기세포배양액 소재의 생물학적 활성에 대해서는 항노화 효과, 조직재생 효과 등 다양한 연구보고가 있으나, 셀인바이오는 피부생리학의 주요 타깃인 히아루론산 생합성 유도 효과를 ADSC-CM 소재 적용을 통하여 확인하고 연구 보고하였다.
Transforming growth factor-beta 1 in adipose derived stem cells conditioned medium is a dominant paracrine mediator determines hyaluronic acid and collagen expression profile
Hana Jung •. Hak Hee Kim •. Dong Hee Lee •.Yu-Shik Hwang •. Hyeong-Cheol Yang •Jong-Chul Park
Springer Science+Business Media B.V. 2010
Abstract
Conditioned medium from adipose derived stem cells (ADSC-CM) stimulates both collagen synthesis and migration of fibroblasts, and accelerates wound healing in vivo. Recently, the production and secretion of growth factors has been identified as an essential function of adipose-derived stem cells (ADSCs). However, the main soluble factor of ADSC-CM which mediates paracrine effects and its underlying mechanism has not been elucidated yet. In this study, we considered transforming growth factor-beta1 (TGF-b1) as a strong candidate for paracrine effect of ADSC-CM and investigated collagen synthesis and hyaluronic acid synthase (HAS) expression. After ADSC-CM addition, collagen type I, type III, HAS and hyaluronic acid (HA) expressions on human dermal fibroblasts (HDFs) were evaluated. Furthermore, to clarify effects of TGF-b1 as a paracrine mediator, TGF-b1 antibody and external supplementary TGF-b1 were treated to HDFs. Collagens type I, type III, HAS-1 and HAS-2 mRNA expressions of HDFs were greatly increased by ADSC-CM treatment, however there was no change in TGF-b1 antibody treated HDFs compared with non-treated control. These results strongly demonstrate that TGF-b1 plays an important role as a paracrine mediator of ECM synthesis. The fact that TGF-b1 contained in ADSC-CM not only accelerates collagen deposition but also increase hyaluronic acid synthesis of HDFs through HAS-1 and HAS-2 expression was also elucidated in this study. Therefore, ADSC-CM shows promise for the treatment of cutaneous wounds and accelerates granulation formation during healing process
Transforming growth factor-beta 1 in adipose derived stem cells conditioned medium is a dominant paracrine mediator determines hyaluronic acid and collagen expression profile
Hana Jung •. Hak Hee Kim •. Dong Hee Lee •.Yu-Shik Hwang •. Hyeong-Cheol Yang •Jong-Chul Park
Springer Science+Business Media B.V. 2010
Abstract
Conditioned medium from adipose derived stem cells (ADSC-CM) stimulates both collagen synthesis and migration of fibroblasts, and accelerates wound healing in vivo. Recently, the production and secretion of growth factors has been identified as an essential function of adipose-derived stem cells (ADSCs). However, the main soluble factor of ADSC-CM which mediates paracrine effects and its underlying mechanism has not been elucidated yet. In this study, we considered transforming growth factor-beta1 (TGF-b1) as a strong candidate for paracrine effect of ADSC-CM and investigated collagen synthesis and hyaluronic acid synthase (HAS) expression. After ADSC-CM addition, collagen type I, type III, HAS and hyaluronic acid (HA) expressions on human dermal fibroblasts (HDFs) were evaluated. Furthermore, to clarify effects of TGF-b1 as a paracrine mediator, TGF-b1 antibody and external supplementary TGF-b1 were treated to HDFs. Collagens type I, type III, HAS-1 and HAS-2 mRNA expressions of HDFs were greatly increased by ADSC-CM treatment, however there was no change in TGF-b1 antibody treated HDFs compared with non-treated control. These results strongly demonstrate that TGF-b1 plays an important role as a paracrine mediator of ECM synthesis. The fact that TGF-b1 contained in ADSC-CM not only accelerates collagen deposition but also increase hyaluronic acid synthesis of HDFs through HAS-1 and HAS-2 expression was also elucidated in this study. Therefore, ADSC-CM shows promise for the treatment of cutaneous wounds and accelerates granulation formation during healing process